1. Introduction to electronic microscopy
Electron-matter interaction
Electron microscope configuration;
Electron sources and electromagnetic lenses
Aberrations and resolutions
Detection systems
Sample preparation methods

2. Scanning electron microscopy
Formation of topographic and compositional images
Low voltage observation modes
Environmental electron microscopy (ETEM)
Scanning electron microscopy (SEM) and nanofabrication: SEM-FIB

3. Transmission electron microscopy
Fundamentals of image formation
Electron diffraction principles
Interpretation of images with diffraction contrast
Phase contrast and high resolution microscopy (HRTEM)
Resolution limits
Interpretation of images with phase contrast

4. Analytical electron microscopy (AEM)
X-ray spectroscopy
Energy dispersion (EDS) and wavelength (WDS) detectors
Qualitative X-ray analysis (XEDS) using SEM and TEM
Introduction to electron energy loss spectroscopy (EELS)

Target group: Any current or future user of the electron microscope facility

Prerequisites: none

Evaluation: Participation in the practical session and assistance to the class up to 80%.
Present the homework requested.

Teaching format: The course will be split in lectures (2x week) and a practical session (1x week). The practical session will be defined depending on the number of participants.

ECTS: 3 Year: 2021

Track segment(s):
BIO-CELL Biology - Cell and Developmental Biology
BIO-MOL Biology - Molecular and Structural Biology
PHY-BIO Physics - Biophysics

Carrie Bernecky Maria Ibáñez Ludek Lovicar Florian Schur

Teaching assistant(s):

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