Microscopy course

The (pre-semester) microscopy course is organized by the (bio-)imaging facility and aims to generate the understanding of how to design your experiments using matching imaging techniques. As a red line we want to raise awareness of the importance of proper data acquisition and the timely image analysis assessments during essay establishment: timely assay-evaluation is critical to determine if the fundamental ‘properties of process of interest’ can be resolved and quantified by the used imaging approach.
To this aim, participants will be introduced to contemporary microscopy techniques, with a focus on the methods that are available at IST Austria. Lectures will cover microscopy-related principles of optics, principles of fluorescence, anatomy of the microscope, methods of optical sectioning, image acquisition methods, super-resolution technologies, basics of electron microscopy & sample preparation, and the basics of data analysis. Due to COVID-19, hands-on sessions are mostly replaced by remote-demonstrations with an interactive nature, and aim to introduce students to the following light microscopy techniques: widefield, TIRF, Confocal, Multiphoton and Spinning disc microscopy, as well as a brief overview of electron microscopy techniques and image processing using FIJI.
Further details: https://bif.pages.ist.ac.at/training-course-materials/microscopy-course/

Target group: Graduate School Students / Post Docs

Prerequisites: Interest in application of microscopy techniques during research project.

Evaluation: written exam + scripting assignment

Teaching format: Lectures, (remote) demonstrations and hands-on.

ECTS: 2 Year: 2020

Teacher(s):
Yann Cesbron Nasser Darwish-Miranda Mohammad Goudarzi Anna Hapek-Sikora Robert Hauschild Gabriel Krens Ludek Lovicar Doreen Milius Judit Singer Christoph Sommer

Teaching assistant(s):

If you want to enroll to this course, please click: REGISTER