This courseProvides conceptual and practical understanding of several of the most advanced techniques in molecular and cellular biology; Encourages students to identify current technical limitations and explore how they can be overcome Topics Basic principles of cloning What is cloning? Introducing the basic vocabulary in cloning Genetics 101 (Promoters and enhancers and why they are so important to us) Design a cloning strategy and perform in silico cloning The underlying principles of non-coding RNA What are non-coding RNAs and how many are existing? Build strategies to profile microRNAs Examine possibilities of verifying functionality of RNAi Design in silico shRNA and clone into a vector Genome editing Discuss various strategies in genome editing How biologists hijacked the bacterial “immune system” CRISPR/Cas in practice: from the biological question to the design of gRNAs Next generation sequencing RNA Seq, FACS sorting, single cell profiling (SMARTSeq versus 10x); ribosome profiling; Whole genome sequencing in organismal and cellular genetics; CRISPR/Cas9 screens Tracking and manipulating proteins and RNA in space and time Fluorescence reporters; halo tag, SNAP tag, RNA tags & uses in cell biology, basic fluorescence microscopy Using epitope tags and nanobodies for cell biology; localizing proteins, targeting them for degradation, adding functionality Chemically induced dimerization and degradation Mass spectrometry Power and limitations of mass spectrometry Co-immunoprecipitation of protein complexes Using Biotin:Streptavidin – proximity labeling with BioID.
Target group: The course is for all students, who wish to affiliate in a Life science lab and have interest in applying molecular and cellular techniques to their research.
Prerequisites: Curiosity, enthusiasm, motivation, and creativity.
Evaluation: Participants are expected to participate actively in discussions during lectures/ recitations, and to solve regular assignments. In the final recitation attendees will be asked to propose a method that extends a protocol discussed in the course. This will involve a 1 – 2 page outline of the method proposed and a 5 minute presentation outlining it.
Teaching format: Lectures will cover the theoretical and practical aspects of a range of methods used in the lab.
Recitations will focus on analyzing papers using certain techniques and discussing the pro’s and con’s of the approaches taken.
ECTS: 3 Year: 2019
BIO-CELL Biology - Cell and Developmental Biology
BIO-MOL Biology - Molecular and Structural Biology
NEU-MOL Neuroscience - Molecular and Cellular Neuroscience
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